Wiktor Koźmiński's NMR group

Biological and Chemical Research Centre, University of Warsaw

  • Increase font size
  • Default font size
  • Decrease font size

New Article in RSC Advances


19F multiple-quantum coherence NMR spectroscopy for probing protein–ligand interactions

Anna Zawadzka-Kazimierczuk, Mate Somlyay, Hanspeter Kaehlig, George Iakobson, Petr Beierd, Robert Konrat


A new 19F NMR method is presented which can be used to detect weak protein binding of small molecules with up to mM affinity. The method capitalizes on the synthetic availability of unique SF5 containing compounds and the generation of five-quantum coherences (5QC). Given the high sensitivity of 5QC relaxation to exchange events (i.e. reversible protein binding) fragments which bind to the target with weak affinity can be identified. The utility of the method in early stage drug discovery programs is demonstrated with applications to two model proteins, the neurotoxic NGAL and the prominent tumor target β-catenin.



Conferences organized

by us:


euromar 2017 logo








We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set.

I accept cookies from this site.