Wiktor Koźmiński's NMR group

Biological and Chemical Research Centre, University of Warsaw

  • Increase font size
  • Default font size
  • Decrease font size

New Article in Journal of Biomolecular NMR

Print

High-dimensionality 13C direct-detected NMR experiments for the automatic assignment of intrinsically disordered proteins

Wolfgang Bermel, Isabella C. Felli, Leonardo Gonelli, Wiktor Koźmiński, Alessandro Piai, Roberta Pierratelli, Anna Zawadzka-Kazimierczuk


alt

We present three novel exclusively heteronuclear 5D 13C direct-detected NMR experiments, namely (HN-flipN)CONCACON, (HCA)CONCACON and (H)CACON(CA)CON, designed for easy sequence-specific resonance assignment of intrinsically disordered proteins (IDPs). The experiments proposed have been optimized to overcome the drawbacks which may dramatically complicate the characterization of IDPs by NMR, namely the small dispersion of chemical shifts and the fast exchange of the amide protons with the solvent. A fast and reliable automatic assignment of α-synuclein chemical shifts was obtained with the Tool for SMFT-based Assignment of Resonances (TSAR) program based on the information provided by these experiments.

 






Banner
Banner
Banner

Conferences organized

by us:


 

euromar 2017 logo

 


 

mmcebanner3

 


 

AUM4

 


We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set.

I accept cookies from this site.